Recombinant Rabbit Anti-NR4A1 Antibody (clone R02-1C7)
CAT#: VS3-FY1068
This product is a recombinant rabbit antibody that recognizes NR4A1. This antibody has been reported for use in Western Blot. The antigen is synthetic peptide of human NUR77. The clone R02-1C7 is specific for rat NR4A1.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/59742/1147_H1_3_selected.jpg
Normal Tissue
Figure 2 IHC staining of human adrenal gland
Immunohistochemical staining of human adrenal gland shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70142/163891_B_5_5_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70142/163891_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70142/163891_A_7_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70142/163891_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70142/163891_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70142/163891_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (HeLa, MCF7, SK-BR-3, SK-MEL-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000123358-NR4A1
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WB
Figure 1. Recombinant Rabbit Anti-NR4A1 Antibody (clone R02-1C7) in WB.
Western Blot analysis of NUR77 in rat brain lysates using NUR77 Antibody.
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Specifications
- Immunogen
- Synthetic peptide of human NUR77.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Rat NR4A1
- Species Reactivity
- Rat
- Clone
- R02-1C7
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- Calculated MW: 64 kDa; Observed MW: 64 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Nuclear Receptor Subfamily 4 Group A Member 1; Nuclear Hormone Receptor NUR/77; Early Response Protein NAK1; Orphan Nuclear Receptor HMR; Orphan Nuclear Receptor TR3; Testicular Receptor 3; NUR77; GFRP1; ST-59; HMR; Nerve Growth Factor IB Nuclear Receptor Variant 1; Nuclear Receptor Subfamily 4, Group A, Member 1
- Gene ID
- 3164
- UniProt ID
- P22736
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Powerful Tool for Cancer Pathway Analysis
The Recombinant Anti-NR4A1 Antibody has strengthened our cancer research protocols, especially for exploring NR4A1's regulatory roles in cell cycle and apoptosis. Its high specificity ensures accurate data, supported by Creative Biolabs' excellent technical support.
Exceptional in Western Blot Applications
The Recombinant Anti-NR4A1 Antibody provides clear and precise results in Western Blot analyses, crucial for detailing NR4A1 expression and interactions, supported by rapid and reliable support services from Creative Biolabs.
Essential for Comprehensive Research Programs
Creative Biolabs' antibody has become an essential part of our toolkit, facilitating broad-spectrum research into NR4A1 functions. Its reproducibility and high-quality standards advance our research initiatives, promising continued success in uncovering disease mechanisms.
Q&As
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What does the NR4A1 antibody target?
A: The Recombinant Anti-NR4A1 Antibody targets the nuclear receptor subfamily 4 group A member 1 (NR4A1), a protein involved in regulating gene expression linked to cell differentiation and apoptosis. It plays a crucial role in inflammatory responses and cancer progression, making it a key focus in related research fields.
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What applications are recommended for this antibody?
A: The antibody is designed for Western Blotting, vital for studying NR4A1's role in cellular contexts and its interactions with other proteins in various pathways.
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What molecular pathways are influenced by NR4A1?
A: NR4A1 is involved in several pathways, including those regulating apoptosis, cellular metabolism, and inflammation. It acts as a transcription factor modulating the expression of genes that are critical for cell survival and immune response.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# VS3-FY1068, RRID: AB_3112058)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "NR4A1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2725z | Mouse Anti-NR4A1 Recombinant Antibody (clone 29B6) | WB, ELISA, IF, IP | Mouse IgG1, κ |
| MOB-2214MZ | Recombinant Mouse Anti-Mouse NR4A1 Antibody (clone 23.25), PE-Conjugated | FC | Mouse antibody |
| MRO-1121-CN | Recombinant Rabbit Anti-NR4A1 Monoclonal Antibody (JM59-11) | WB, IF, IHC | Rabbit IgG |
| VS3-WK596 | Rabbit Anti-NR4A1 Recombinant Antibody (clone JM59-11) | WB, ICC, IF, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1790CQ | Recombinant Mouse Anti-NR4A1 Antibody (NEUT-1790CQ) | Neut, FC | IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2494 | Hi-Affi™ Recombinant Rabbit Anti-NR4A1 Monoclonal Antibody (DS2494AB) | WB, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1516 | Anti-NR4A1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4963 | Anti-Human NR4A1 Immunohistochemistry Kit | IHC |
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